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8506856 
Journal Article 
Establishment of tripterygium wilfordii hook. f. hairy root culture and optimization of its culture conditions for the production of triptolide and wilforine 
Zhu, C; Miao, G; Guo, J; Huo, Y; Zhang, X; Xie, J; Feng, J 
2014 
Yes 
Journal of Microbiology and Biotechnology
ISSN: 1017-7825
EISSN: 1738-8872 
Korean Society for Microbiolog and Biotechnology 
SEOUL 
24 
6 
823-834 
English 
24651642 
WOS:000338177600013 
In order to solve the shortage of natural Tripterygium wilfordii Hook. f. plant resource for the production of the important secondary metabolites triptolide and wilforine, hairy roots were induced from its root calli by Agrobacterium rhizogenes. Induced hairy roots not only could be maintained and grown well in hormone-free half-strength Murashige and Skoog medium but also could produce sufficient amounts of both triptolide and wilforine. Although hairy roots produced approximately 15% less triptolide than adventitious roots and 10% less wilforine than naturally grown roots, they could grow fast and could be a suitable system for producing both secondary metabolites compared with other tissues. Addition of 50 μM methyl jasmonate (MeJA) could slightly affect hairy root growth, but dramatically stimulated the production of both triptolide and wilforine, whereas 50 μM salicylic acid had no apparent effect on hairy root growth with slightly stimulatory effects on the production of both secondary metabolites. Addition of precursor nicotinic acid, isoleucine, or aspartic acid at the concentration of 500 μM had varying effects on hairy root growth, but none of them had stimulatory effects on triptolide production, and only the former two had slightly beneficial effects on wilforine production. The majority of triptolide produced was secreted into the medium, whereas most of the produced wilforine was retained inside of hairy roots. Our studies provide a promising way to produce triptolide and wilforine in T. wilfordii hairy root cultures combined with MeJA treatment. © 2014 by The Korean Society for Microbiology and Biotechnology. 
Agrobacterium rhizogenes; Hairy roots; Methyl jasmonate; Tripterygium wilfordii; Triptolide; Wilforine