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8551096 
Journal Article 
新型亲水性荧光探针BODIPY-PC-RGD的制备及性能测试 
Meng, S; Meng, Y; Xue, Z; Feng, T; Feng, Y 
2020 
Tianjin University 
53 
3 
317-323 
Chinese 
First, 2, 4-dimethylpyrrole and carbazole were used as the starting materials to synthesize the BODIPY framework and 4-diarylamino formaldehyde derivative, respectively. The strong electron-donating groups were introduced to the C-3, 5 position of the framework by the Knoevenagel reaction, contributing to a new fluorochrome whose emission wavelength can be tuned to 680 nm. Subsequently, the long chain (DSPE-PEG2000-MAL) was connected to the target group RGD with thiol group to form an amphiphilic compound (DSPE-PEG2000-MAL-RGD). Then, BODIPY was self-assembled with DSPE-PEG2000-MAL-RGD to form nanoparticles (BODIPY-PC-RGD), which could be targeted to improve the water solubility and biocompatibility of BODIPY. The structure of BODIPY was confirmed by 1HNMR, and its properties were tested by fluorescence microscopy, SEM, and TEM. After self-assembly, the nanoparticles were approximately 143 nm in size and had a uniform morphology. The properties of the fluorescent probe were evaluated by cytotoxicity, co-focusing, and flow cytometry experiments. The results showed that the probes exhibited weak, even negligible, cytotoxicity to the U87 and Hela cells at low concentration. When the cells were treated with BODIPY-PC-RGD, more probes could penetrate the cells, which were observed from the fluorescent areas in the graphs. The results indicated that BODIPY-PC-RGD possesses the capability of targeting cancer cells to some extent, showing its long-term development potential in the field of tumor detection and diagnosis in the future. © 2020, Editorial Board of Journal of Tianjin University(Science and Technology). All right reserved. 
BODIPY; Fluorescent probe; Lecithin; RGD