Xia, HM; Qu, YW; Wang, L; Guo, W; Zhang, DM
Objective: To investigate the chemical constituents and hepatoprotective effects from the stems of Clausena emarginata. Methods: The compounds were isolated and purified by chromatography on kieselguhr, silica gel, MPLC, and preparative HPLC. Their structures were identified based on physicochemical properties and spectral data. Hepatoprotective activities against DL-galactosamine-induced damage and inhibitory effects on LPS-induced NO production of the compounds were investigated. Results: Eleven compounds were isolated from the CHCl3 fractions of 95% ethanol extract of the stems of C. emarginata, and their structures were identified as nordentatin (1), oxanordentatin (2), anisocoumarin H (3), 7-((E)-7'-hydroxy-3',7'-dimethylocta-2', 5'-dienyloxy)-coumarin (4), 7-hydroxycoumarin (5), claulamine A (6), γ-fagarine (7), secoisolariciresinol (8), 2-{4-[(1E)-3- hydroxyprop-1-en-1-yl]-2,6-dimethoxyphenoxy}propane-1,3-diol (9), 2-[4-(3-hydroxy-1-propenyl)-2-methoxyphenoxy]-1,3- propanediol (10), and methyl 2-O-β-D-glucopyranosylbenzoate (11). Compounds 1-5 were coumarins, 6 was a carbazole alkaloid, 7 was a furoquinoline alkaloid, 8 was a lignan, 9-10 were phenylpropanoids, and 11 was a phenolic acid. Conclusion: Compounds 2-4, 7-11 are isolated from this plant for the first time. Compounds 5 and 7 show hepatoprotective effects against DL-galactosamine-induced toxicity in WB-F344 cells. Compound 11 shows inhibitory effect against LPS-induced NO production in microglia BV2 Cell. © 2021, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.