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8565019 
Journal Article 
Determination of Guaiazulene in Cosmetics by High Performance Liquid Chromatography 
Kamata, K; Kan, T; Harada, H 
1984 
1 
Eisei Kagaku / Journal of Hygienic Chemistry
ISSN: 0013-273X 
PHARMACEUTICAL SOC JAPAN 
TOKYO 
30 
3 
144-148 
English 
WOS:A1984TA59000006 
A procedure is described for the determination of guaiazulene (GA) in cosmetics by high performance liquid chromatography (HPLC). The sample was mixed with Cellite, transfered to a chromatographic column, and eluted with acetOnitrile. The acetonitrife was added to water, and liquid-liquid partition between hexane. The hexane was concentrated and then GA was determined by HPLC on octadecylsilated silica column using a mobile phase of methanol-water (9: 1, v/v), UV detector (284 nm) and fluorescence spectrophotometric detector (FD) (Ex: 285 nm, Em-/i: 390 nm) for detection. As a result, the calibration curve was linear in the range 1-10 ngfmX of GA on FD and UV detector. Recoveries of GA obtained from a variety of cosmetic samples spiked at 50 and 100 ppm levels were 93. 8-97. 2 % and 94. 8-97. 0 %, respectively. When this method was applied to the analysis of GA in commercial samples, GA could be determined without the influence of obstructive substances. © 1984, The Pharmaceutical Society of Japan. All rights reserved. 
celite column; cosmetic; guaiazulene; guaiazulene determination; HPLC