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8572099 
Journal Article 
Simultaneous analysis of L-carnitine, acetyl-L-carnitine and propionyl-L-carnitine in human plasma by HPLC 
Sun, YX; Lu, CX; Tang, QL; Cao, Y; Xue, YZ; Wan, XX; Wang, CB 
2007 
0 
Zhongguo Yaoxue Zazhi
ISSN: 1001-2494 
42 
18 
1425-1428 
Chinese 
OBJECTIVE To establish a new sensitive method for the simultaneous determination of L-carnitine(LC), Acetyl-L-carnitine (ALC) and Propionyl-Lcarnitine (PLC) in Human Plasma by HPLC. METHODS The analytes were extracted by protein precipitation and then precolumn derivatization with 1-aminoanthracene (IAA) was performed. The fluorescent derivatives were separated on a Hypersil C18 column, and the mobile phase consisted of acetonitrile-0. 1 mol • L-1 ammonium acetate(28: 72), the flow rate was 1. 0 mL • min-1. The derivatives were monitored with a fluorescent detector set at 248 nm (excitation wavelength) and 418 nm (emission wavelength). RESULTS The good linearity was observed over the concentration ranges of 5 ∼ 160 μmol • L-1 for LC, 1 ∼ 32 μmol • L-1 for ALC, 0. 25 ∼ 8 μmol • L-1 for PLC. The recoveries of LC, ALC and PLC were 99. 48%, 99. 94% and 98. 20%, respectively. The intra- and inter-day validation were adequate with the RSDs of 5. 83% or below. CONCLUSION This method is proved to be stable, accurate and convenient to study the concentrations of LC, ALC and PLC in plasma. 
Acetyl-L-carnitine; HPLC; L-carnitine; Propionyl-L-carnitine