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8586005 
Journal Article 
Spectrophotometric determination of trace Hg (II) using 6-(anthracen-2-yl)-2, 3- dihydro -1, 2, 4- triazine-3-tione as a newly synthesized reagent 
Tehrani, MB; Forumadi, A; Hashem Shamsa, S; Mojtahedi, E 
2012 
0 
Research Journal of Pharmaceutical, Biological and Chemical Sciences
ISSN: 0975-8585 
3 
4 
65-72 
English 
A simple and accurate spectrophotometric method for Hg determination with a newly synthesized reagent, 6-(2-anthracenyl) - 2, 3-dihydro-1, 2, 4-triazine-3-thione (ADTT), as a selective analytical reagent has been developed. Determination of trace Hg (II) in environmental and industrial samples was of great importance because of its health hazard for humans. Many analytical methodologies have been proposed for determination of Hg based on atomic absorption spectroscopy and HPLC. Although these methods are selective and sensitive, they have some disadvantages, requiring expensive equipment and toxic solvents, and often involving complex sample pre-treatments. Spectrophotometric methods were suitable for routine laboratories. In this paper we reported the synthesis of a new reagent, 6-(2-anthracenyl) - 2, 3-dihydro-1, 2, 4-triazine-3-thione (ADTT), and its applicability for Hg determination. 2-acethylanthracene was synthesized selectively according to the reaction of anthracene with acethylchloride under the special condition. Use of amyl nitrite and 2-acethylanthracene under anhydrous condition 2-)anthracen-2-yl) oxoacetaldehyde oxime produced, which upon reaction with thiosemicarbazone yielded 6-(2-anthracenyl)- 2,3-dihydro-1,2,4-triazine-3-thione. The yellow complex of Hg(II) with ADTT was formed in basic media according to a 1:3 stoichiometry. Beer's law was obeyed in the Hg concentration range of 5-30μg/ ml. The linear regression equation were A= 0.010C - 0.014 and A= 0.023C - 0.010 in ymax 330nm and 254nm respectively. The limit of detection was 1 μg/ml. The relative standard deviations of 10 replicate determinations were 2.53% and 0.7% for determination of 4.5 and 16 μg/ml of Hg respectively. The method was applied to the determination of Hg (II) in amalgam sample with satisfactory results. 
6-(2-anthracenyl) - 2, 3-dihydro-1, 2, 4-triazine-3-thione; Mercury (II); Uv-vis spectrophotometry