US EPA

8586053 

Journal Article 

Dose response study of Agaricus Bisporus (white button mushroom) and its encapsulated chitosan nanoparticles against 7,12-imethylbenz(A)anthracene induced mammary carcinogenesis in female Sprague-Dawley rats 

Dhamodharan, G; Mirunalini, S 

2012 

1 

International Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 2656-0097
EISSN: 0975-1491 

4 

4 

348-354 

English 

Purpose: The present study was aimed to investigate the comparative dose response effect of Agaricus bisporus (AB) and Agaricus bisporus loaded chitosan nanoparticles (ABCNPs) against 7, 12-dimethylbenz(a)anthracene (DMBA) induced mammary carcinogenesis in female Sprague-Dawley rats. Materials and Method: A single subcutaneous injection of DMBA (25 mg/kg bw) in the mammary gland was induced to develop mammary carcinoma on experimental animals. Rats were treated with different doses of AB (100, 200 & 400 mg/kg bw) and ABCNPs (15, 30 & 60 mg/kg bw) which effectively reduced the oxidative damage on DMBA induced tumor bearing animals, which was revealed by decrease in the extent of lipid peroxidation with concomitant increase in the activities of enzymatic antioxidants (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)) and non-enzymatic (reduced glutathione (GSH), Vitamin C and Vitamin E) levels on experimental animals. Results: From our results we concluded that among different doses of treatment, 200 mg/kg bw of AB and 30 mg/kg bw of ABCNPs were found to be the optimum dose which effectively altered the DMBA induced oxidative stress on mammary carcinogenesis. Conclusion: The oral administration of AB and ABCNPs has dose dependently inhibited the DMBA induced mammary carcinogenesis in experimental animals. 

Agaricus bisporus; Chitosan nanoparticles; DMBA; Lipid peroxidation; Nanoparticles; Sprague dawley rats