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8588489 
Journal Article 
Butyl Esterification for Determination of Organic Acids in Sugars 
Saito, S; Miki, T; Ito, H; Kamoda, M 
1983 
1 
Nippon Nogeikagaku Kaishi
ISSN: 0002-1407
EISSN: 1883-6844 
AGR CHEM SOC JAPAN 
TOKYO 
57 
2 
147-149 
English 
WOS:A1983QE87000006 
Aqueous solution containing about 2 mg of organic acid was neutralized with 1n-NaOH, and the solution was evaporated to dryness under reduced pressure at 40°C. Five milliliters of n-butanol, 0.7 g of Amberlite CG 120 (H form, 200~300 mesh) and 2g of anhydrous sodium sulfate were added to a flask containing the dried material, and the mixture was refiuxed for 2 hours. After esterification, 3~5 ml of n-hexane was added and filtered through a filter paper. The filtrate was subjected to GLC analysis. This esterification procedure was simple and rapid. © 1983, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.