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8588875 
Journal Article 
Determination of Gossypol in Edible Cottonseed Oil by High Performance Liquid Chromatography 
Yabe, Y; Tan, S; Ninomiya, T; Okada, T 
1984 
1 
Shokuhin Eiseigaku Zasshi / Journal of the Food Hygienic Society of Japan
ISSN: 0015-6426
EISSN: 1882-1006 
FOOD HYG SOC JPN 
TOKYO 
25 
3 
264 
English 
WOS:A1984SX67800007 
A method was developed for the determination of residual gossypol in edible cottonseed oil by high performance liquid chromatography. Gossypol in cottonseed oil was dissolved in w-hexane containing a little tetrahydrofuran, and then extracted with acetonitrile. The acetonitrile layer was concentrated and made up to volume with tetrahydrofuran. The test solution was injected into a high performance liquid chromatograph. Recoveries of gossypol added to cottonseed oil at levels of 50 and 200 μg/10 g were 87. 4 and 93. 7%, respectively. In heating experiments (180°C for 5, 15 and 30 minutes), it was found that the amount of gossypol added to cottonseed oil decreased with heating time. By this method, no gossypol (less than 1 μg/g) was detectable in commercial edible cottonseed oil. © 1984, Japanese Society for Food Hygiene and Safety. All rights reserved. 
edible cottonseed oil; gossypol; high performance liquid chromatography