US EPA

8624982 

Journal Article 

Anticryptococcal activity of alkaloid rich fraction of leaves of Prosopis juliflora - A future promising supplementary therapy for cryptococcosis and cryptococcal meningitis? 

Valli, S; Gokulshankar, S; Mohanty, BK; Ranjith, MS; Ashutosh, SR; Remya, V 

2014 

1 

International Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 2656-0097
EISSN: 0975-1491 

IJPPS 

6 

2 

491-495 

English 

Objective: To study the antifungal activity of alkaloids from leaves of Prosopis juliflora against clinical and environmental isolates of Cryptococcus neoformans and to identify the bioactive compounds by GC MS FTIR. Methods: The alkaloid rich fraction obtained from the leaf of Prosopis juliflora was assessed for their antifungal activity against clinical and environmental isolates of Cryptococcus neoformans. Alkaloids were separated from the leaves by standard techniques and HPTLC profile of the alkaloids was performed. The alkaloids were identified by GC MS.analysis and the functional groups were identified by FTIR. Results: Of the 10 isolates tested the clinical isolates were more susceptible than the environmental isolates. Isolate C3 was found to be more susceptible with the zone of inhibition 17mm at a concentration of 200 μg. Mass spectrometric analysis revealed the presence of piperidine alkaloids. FTIR analysis was performed to identify the chemical groups constituting the bioactive compounds. Conclusion: Alkaloids from leaf extract of Prosopis juliflora was found to possess antifungal activity and have the potential to inhibit drug resistant fungal strains.GC MS analysis revealed that leaves of Prosopis juliflora are rich source of piperidine alkaloids. 

Antifungal; Cryptococcal meningitis; Cryptococcus neoformans; Piperidine; Prosopis juliflora