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8625099 
Journal Article 
Antifungal mechanism of Streptomyces ma. FS-4 on fusarium wilt of banana 
Duan, Y; Chen, J; Pang, Z; Ye, X; Zhang, C; Hu, H; Xie, J 
2021 
Yes 
Journal of Applied Microbiology
ISSN: 1364-5072
EISSN: 1365-2672 
Blackwell Publishing Ltd 
130 
1 
196-207 
English 
Aim: Research on prevention and cure of banana wilt is important to ensure the healthy development of the banana industry. In this study, antifungal mechanism of Streptomyces ma. FS-4 on fusarium wilt of banana was investigated. Methods and Results: The physiological strain of banana fusarium pathogen Fusarium oxysporum f. sp. cubense Race 4 (FOC.4) was used as the target fungus, and the antifungal mechanism of the crude extract of Streptomyces ma. FS-4 was investigated. Eighteen different compounds identified by gas chromatography–mass spectrometry were composed of aldehydes, methyl, hydrocarbons, amides, esters and acids. FS-4 significantly inhibited the spore germination of the target fungi, with an EC50 of 22·78 μg ml−1. After treatment with 100 μg ml−1 FS-4 crude extract, the N-acetylglucosamine content in the mycelium increased 1·95-fold. However, the extract had no significant effect on β-1,3-glucanase. At the FS-4 crude extract dose of 100 μg ml−1, the total sugar and protein contents decreased by 28·6 and 29·1% respectively, and the fat content was 41·3%. FS-4 significantly inhibited the activity of the mitochondrial complex III of Foc4, which was reduced by 52·45%. Moreover FS-4 reduced the activity of succinate dehydrogenase, a key enzyme in the Krebs cycle, by 60·2%. However, FS-4 had no significant effect on malate dehydrogenase. The membrane potential on the mitochondrial inner membrane was significantly reduced at the test concentration of 100 μg ml−1. ROS gradually accumulated in the Foc4 hypha, and the burst was 3·97 times higher than the control. Conclusions: This study demonstrated that the antifungal mechanism of Streptomyces ma. FS-4 against Foc4 includes the destruction of the plasma membrane and mitochondrial dysfunction and finally induction of cell apoptosis. Significance and Impact of the Study: These results may indicate the prevention and control of banana wilt, which is of great significance to the healthy development of banana industry system. © 2020 The Society for Applied Microbiology 
antimicrobials; cell injury; mechanism of action; plant diseases; Streptomycetes