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8626018 
Journal Article 
Preparation and characterization of Black Phosphorus Nanosheets (BPNSs) and its toxic effects on Scenedesmus Obliquus 
Li, Z; Li, H; Li, Y; Wang, M; Mo, F; Kong, Y; Nan, R; Xu, J 
2021 
Yes 
Huanjing Kexue Xuebao / Acta Scientiae Circumstantiae
ISSN: 0253-2468 
41 
6 
2448-2456 
Chinese 
Black Phosphorus nanosheets(BPNSs)has a wide range of application prospects, whereas research regarding safety of BPNSs has been less investigated. BPNSs with the thickness of (58.05±36.48) nm and the lateral size of (541.25±176.22) nm were prepared by liquid exfoliation in this study in order to explore the toxic effect of BPNSs on aquatic organisms. The primary producer Scenedesmus Obliquus was selected and exposed to 0, 10, 50, and 100 mg•L-1 BPNSs to study the toxic effects. The results showed that BPNSs inhibited the growth of Scenedesmus Obliquus at all concentrations and the EC50 was 74.86 mg•L-1 at 120 h. The content of Chlorophyll A in three treatment groups markedly decreased and showed a dose-effect, indicating that BPNSs caused damage to the photosynthetic system of Scenedesmus Obliquus. The content of reactive oxygen species (ROS) in three treatment groups increased significantly after 48 h exposure, indicating the occurrence of oxidative damage mediated by BPNSs. BPNSs wrapped algae cells were observed using microscope, leading to the speculation that BPNSs may aggregate on the surface of Scenedesmus Obliquus by adsorption. Physical damage in Scenedesmus Obliquus caused by BPNSs was verified utilizing FE-SEM which showed the destroyed cell morphology. This research can provide theoretical support for the subsequent studies on the potential enrichment risk of BPNSs in the water environmental food chain. © 2021, Science Press. All right reserved. 
Adsorption agglomeration; Black phosphorus nanosheets; Oxidative damage; Physical damage; Scenedesmus Obliquus