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8627614 
Journal Article 
基于Caco-2细胞单层模型的二硫化钨纳米材料肠毒性研究 
Gu, W; Wu, X; Liu, S; Wu, B 
2021 
Yes 
Huanjing Kexue Xuebao / Acta Scientiae Circumstantiae
ISSN: 0253-2468 
Science Press 
41 
5 
2039-2046 
Chinese 
Tungsten disulfide (WS2) as a two-dimensional nanomaterial has a broad application prospect in the fields of electronics, energy and medicine due to its unique crystal structure and mechanical flexibility. In this paper, human colon cancer Caco-2 cells were used to construct an in vitro intestinal absorption model, and the toxic effects of WS2 on intestinal cell layer structure and function were studied. Results showed that 1~80 μg•mL-1 WS2 did not induce the change of mitochondrial membrane potential in the Caco-2 cells, and ≥ 50 μg•mL-1 WS2 increased the content of intracellular reactive oxygen species, showing low cytotoxicity. For the monolayer of Caco-2 cells, 2 and 20 μg•mL-1 WS2 did not change their permeability, integrity, and expression of genes related to oxidative stress. However, WS2 inhibited the activity of plasma membrane ABC transporter in the Caco-2 cell monolayer. When the WS2 were co-exposed to the Caco-2 cell monolayer with cadmium, arsenic, benzo(a)pyrene, the cell membrane integrity and monolayer transport function in the monolayer did not change, however, the influence of cadmium on the expressions of oxidative stress-related genes GPx and OXSR1 in the cell monolayer increased. Results of this study provide basic data for the evaluation of gastrointestinal toxicity and health risk of WS2. © 2021, Science Press. All right reserved. 
Caco-2 cells; Combined toxicity; Intestinal toxicity; Tungsten disulfide