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8633310 
Journal Article 
Preparation and Characterization of Antioxidant Peptides from Fermented Goat Placenta 
Hou, Y; Zhou, J; Liu, W; Cheng, Y; Wu, L; Yang, G 
2014 
Yes 
Han-gug Chugsan Sigpum Hag-hoeji
ISSN: 1225-8563 
34 
6 
769-776 
English 
26761673 
WOS:000347514000007 
The goat placenta was fermented by Bacillus subtilis and the optimal fermentation parameters of strongest antioxidant capacity of peptides were obtained using response surface methodology (RSM). The effects of fermentation time, initial pH value and glucose content on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity of the goat peptides were well fitted to a quadric equation with high determination coefficients. According to the data analysis of design expert, the strongest DPPH radical scavenging capacity value was obtained with the following conditions: content of glucose was 2.23%, initial pH value was 7.00 and fermentation time was 32.15 h. The DPPH radical scavenging capacity commonly referring antioxidant activity showed a concentration dependency and increased with increasing peptide concentration. The effects of temperature and pH were assessed to determine the stability of antioxidant peptides prepared from goat placenta. Antioxidant peptides showed good stabilities when temperature was lower than 70℃. However, the antioxidant peptides lost antioxidant activities rapidly under alkaline and excessive acid condition. Ultrafiltration technique was performed to separate fermentation broth with different Mw (molecular weight). It was found that peptides in the range of < 3 KDa mainly accounted for the antioxidant activities. 
Bacillus subtilis; goat placenta; antioxidant; peptide; fermentation