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HERO ID
8633310
Reference Type
Journal Article
Title
Preparation and Characterization of Antioxidant Peptides from Fermented Goat Placenta
Author(s)
Hou, Y; Zhou, J; Liu, W; Cheng, Y; Wu, L; Yang, G
Year
2014
Is Peer Reviewed?
Yes
Journal
Han-gug Chugsan Sigpum Hag-hoeji
ISSN:
1225-8563
Volume
34
Issue
6
Page Numbers
769-776
Language
English
PMID
26761673
DOI
10.5851/kosfa.2014.34.6.769
Web of Science Id
WOS:000347514000007
Abstract
The goat placenta was fermented by Bacillus subtilis and the optimal fermentation parameters of strongest antioxidant capacity of peptides were obtained using response surface methodology (RSM). The effects of fermentation time, initial pH value and glucose content on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity of the goat peptides were well fitted to a quadric equation with high determination coefficients. According to the data analysis of design expert, the strongest DPPH radical scavenging capacity value was obtained with the following conditions: content of glucose was 2.23%, initial pH value was 7.00 and fermentation time was 32.15 h. The DPPH radical scavenging capacity commonly referring antioxidant activity showed a concentration dependency and increased with increasing peptide concentration. The effects of temperature and pH were assessed to determine the stability of antioxidant peptides prepared from goat placenta. Antioxidant peptides showed good stabilities when temperature was lower than 70℃. However, the antioxidant peptides lost antioxidant activities rapidly under alkaline and excessive acid condition. Ultrafiltration technique was performed to separate fermentation broth with different Mw (molecular weight). It was found that peptides in the range of < 3 KDa mainly accounted for the antioxidant activities.
Keywords
Bacillus subtilis; goat placenta; antioxidant; peptide; fermentation
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