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8645972 
Journal Article 
UPLC-MS/MS快速测定烟草中2,4-滴和2,4,5-涕 
Yang, F; Liu, S; Deng, H; Bian, Z; Li, Z; Fan, Z; Wang, Y; Tang, G 
2019 
Editorial Office of Tobacco Science and Technology 
52 
1 
60-66 
Chinese 
In order to analyze two common chlorinated phenoxy acid residues in tobacco, an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for quantifying 2,4-D and 2,4,5-T was proposed. The analytes were extracted from tobacco samples by a hydrochloric acid solution and CH2Cl2. The extract was analyzed qualitatively and quantitatively by a tandem mass spectrometer in multiple reaction monitoring (MRM) mode after being separated on C18 column. The results showed that: 1) A good linear relation was obtained in the range of standard working solution with r>0.999. The limits of detection (LODs) and quantification (LOQs) were 0.004 5-0.007 6 and 0.013 8-0.023 6 mg/kg, respectively. The added standard recoveries of blank samples ranged from 79.5% to 94.6% with the relative standard deviations less than 6.0%. 2) The method was applied to 30 different types of tobacco samples, the results agreed well with those obtained by the tobacco industry standard method based on GC-MS. The proposed method is rapid and sensitive, no derivatization required, and consumes less organic reagents. It is suitable for the analysis of 2,4-D and 2,4,5-T in flue-cured tobacco, burley tobacco and oriental tobacco. © 2019 Editorial Office of Tobacco Science and Technology. All right reserved. 
2,4,5-T; 2,4-D; Chlorinated phenoxy acid residue; Tobacco; Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)