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8649937 
Journal Article 
Oxidative stress damage from nanometer titanium dioxide in goldfish brain tissue 
Sun, H; Zhang, Q; Chen, S; Cao, J 
2015 
Chk 
Huanjing Kexue Yanjiu / Research of Environmental Sciences
ISSN: 1001-6929 
28 
6 
930-935 
Chinese 
In order to investigate the oxidative damage mechanism of photocatalytic type nano-TiO2 on goldfish brain tissue, a test involving the content of malondialdehyde (MDA), protein carbonyl (PC) and H2O2 in goldfish brain tissues together with the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) and the expression of Cu/Zn-SOD protein was determined after goldfishes were exposed to different nano-TiO2 concentrations of 0, 16, 32, 64 and 128 mg/L. The results showed that the Cu/Zn-SOD activity in goldfish brain tissues decreased from 1.70×10-3 kat/kg to 0.35×10-3 kat/kg, and then rebounded to 0.46×10-3 kat/kg with the nano-TiO2 concentration varying from 0 to 128 mg/L. Meanwhile, the Mn-SOD activity presented a decrease-increase-decrease trend; the MDA content increased from 0.85 to 2.83 nmol/g, and then decreased to 2.54 nmol/g. The PC content increased all the way when the H2O2 content increased from 4.32 to 9.28 μmol/g, and then decreased to 8.38 μmol/g. When the nano-TiO2 concentration increased, the expression of Cu/Zn-SOD decreased gradually. The research indicated that nano-TiO2 strongly affected the oxidative damage in goldfish brain tissues. The increase of the MDA and PC content was associated with the decreasing of the Cu/Zn-SOD and GPx activity, while H2O2 has a bearing on the GPx activity and other factors. ©, 2015, Editorial department of Molecular Catalysis. All right reserved. 
Glutathione peroxidase; Malondialdehyde; Nanometer titanium dioxide; Oxidative damage; Protein carbonyl; Superoxide dismutase