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8652803 
Journal Article 
Effect of manganese on Caspase-3 mRNA regulation in spermatogenic cell and the expression of vimentin on Sertoli cell in rats 
Cai, XL; Wang, GX; Guo, H 
2010 
0 
Jiepou Xuebao
ISSN: 0529-1356 
41 
3 
400-404 
Chinese 
Objective: To determine the effect of manganese on Caspase-3 mRNA regulation in spermatogenic cell and the expression of vimentin on Sertoli cell in rat. Methods: Forty-eight male rats were randomly divided into one control group and two manganese chloride (MnCl2) (15mg/kg, 30 mg/kg) groups. After exposed to manganese for 4 and 6 weeks respectively, the apoptosis of spermatogenic cell was examined by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) technique. The expression of Caspase-3 mRNA in spermatogenic cells was investigated by in situ hybridization, and the expression of vimentin on Sertoli cell of rats was detected by immunohistochemitry (SABC). Results: 1. Compared with control groups, AI was increased (P < 0.05, P < 0.01), the Caspase-3 mRNA-positive-cell rate was increased significantly (P < 0.01), the vimentin-positive-cell rate was decreased significantly (P < 0.01) in every manganese exposure groups. 2. Among manganese exposure groups of same dose, AI and the Caspase-3 mRNA-positive-cell rate were increased more significantly, but the vimentin-positive-cell rate was decreased more significantly in 6 weeks manganese exposure groups than that in 4 weeks manganese exposure groups (P < 0.01). 3. Among manganese exposure groups of same time, AI and Caspase-3 mRNA-positive-cell rate in 30mg/kg manganese exposure groups were increased more significantly than that in 15mg/kg manganese exposure groups (P < 0.01), but the vimentin-positive-cell rate was decreased more significantly in 30 mg/kg manganese exposure groups than that in 15 mg/kg manganese exposure groups (P < 0.01). 4. There existed a positive correlation between AI and the Caspase-3 mRNA-positive-cell rate (r = 0.842, P < 0.01), a negative correlation between AI and vimentin-positive-cell rate (r = -0.859, P < 0.05), and a negative correlation between the Caspase-3 mRNA-positive-cell rate and the vimentin-positive-cell rate (r = -0.975, P < 0.01). Conclusion After exposed to manganese the increased expression of Caspase-3 mRNA in spermatogenic cells led to increased apoptosis of spermatogenic cell and decreased expression of vimentin Sertoli cell in rats, which might be one of the most important molecular mechanisms of reproductive toxicity of manganese. 
Caspase-3 mRNA; Immunohistochemistry; In situ hybridization; Manganese; Spermatogenic cell; Vimentin