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Citation
Tags
HERO ID
8668794
Reference Type
Journal Article
Title
Detection of LINE RT elements in the olive flounder (Paralichthys olivaceus) genome and expression analysis after infection with S-parauberis
Author(s)
Gim, JAn; Nam, GyuHwi; Kim, WooJin; Lee, HeeEun; Mishra, A; Choe, Y; Park, CIl; Kim, DoH; Choi, YH; Cha, HeeJae; Yoon, D; Kim, S; Kim, HSoo
Year
2016
Is Peer Reviewed?
Yes
Journal
Genes and Genomics
ISSN:
1976-9571
Volume
38
Issue
11
Page Numbers
1105-1110
DOI
10.1007/s13258-016-0457-8
Web of Science Id
WOS:000385159500010
Abstract
Long interspersed nuclear elements (LINEs) are widely distributed in the vertebrate genome, and can be either beneficial or detrimental to the host genome. Here we identified three members of LINE RT elements in the olive flounder genome. They showed high amino acid sequence identity (89-99 %), and the sequences of LINE reverse transcriptase (RT) in olive flounder are closely related to those of coral grouper, European seabass, and three-spined stickleback. Real-time reverse transcription-polymerase chain reaction analysis indicated that expression of the OF (Olive flounder)-LINE Chr3-1 RT increased more in spleen than in other tissues after treatment with the pathogen Streptococcus parauberis. These data may form the basis for further studies on the function of retroelements in infected olive flounder.
Keywords
Expression; Long interspersed nuclear elements; Olive flounder; Paralichthys olivaceus; Streptococcus parauberis
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