US EPA

8688337 

Journal Article 

Enzyme potentiated hyposensitization III: Control by sugars and diols of the immunological effect of β glucuronidase in mice and patients with hay fever 

Mcewen, LM; Nicholson, M; Kitchen, I; White, S 

1973 

Yes 

Annals of Allergy
ISSN: 0003-4738 

31 

11 

543-550 

English 

Mice previously sensitized to horse serum were treated with a mixture of β glucuronidase and a small dose of horse serum. One week later the animals were challenged by pinnal anaphylaxis. The changes in anaphylactic responsiveness produced by the treatment with antigen and enzyme could be controlled by the addition to the mixture of small doses of various pyranose derivatives, ascorbic acid, heparin or a number of diols. Among the compounds tested a 1,3 diol grouping appeared to confer the greatest activity. The dose response curve for these substances was W shaped; two doses induced hyposensitization while an intervening dose caused hypersensitization. Using aliquots of β glucuronidase stored with 1,3 cyclohexane diol, it was shown that the diol's effect was not lost after storage for 10 weeks at +4°C. Patients sensitive to grass pollen were treated with a mixture of grass pollen extract, hyaluronidas and further aliquots of β glucuronidase stored with differing doses of 1,3 cyclohexane diol. Subsequently, they were challenged with intranasal grass pollen extract. The responses to challenge of groups of subjects treated with different doses of diol parallelled the W shaped dose response curve from the mouse experiment; patients treated with β glucuronidase stored with 10-9 gm/ml of the diol were significantly hypersensitized. Patients treated with β glucuronidase which had been stored with 10-6 and 10-10 gm/ml of the diol reacted to challenge less than the saline treated controls but the results did not reach statistical significance.