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8703602 
Journal Article 
Conformation and immunochemistry of parvalbumin III from Pike white muscle. Modification of the arginine residue with 1,2-cyclohexanedione 
Gosselin-Rey, C; Bernard, N; Gerday, C 
1973 
303 
1 
90-104 
English 
1. 1. The single arginine residue of parvalbumin III from Pike white muscle has been modified with 1,2-cyclohexanedione. The purity of the modified protein is established by amino acid analysis, immunoelectrophoresis and peptide mapping. 2. 2. The immunochemical study indicates the loss of at least two antigenic determinants together with conformational changes in the molecule. 3. 3. A reduced percentage of α helicity is demonstrated by ORD and CD measurements. 4. 4. The Ca2+ associated with the modified protein is reduced to 50-25% of the amount in the native parvalbumin. 5. 5. It is concluded that the arginine residue plays a key role in the tertiary structure of the molecule. 1973.