Gradient elution in liquid chromatography. III. Verification of the theoretical relationships for elution characteristics (retention volume, band width and resolution) in isocratic and gradient elution chromatography on silica | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

8703701

Reference Type

Journal Article

Title

Gradient elution in liquid chromatography. III. Verification of the theoretical relationships for elution characteristics (retention volume, band width and resolution) in isocratic and gradient elution chromatography on silica

Author(s)

Jandera, P; Churáček, J

Year

1974

Is Peer Reviewed?

Yes

Journal

Journal of Chromatography A
ISSN: 0021-9673
EISSN: 1873-3778

Volume

Issue

Page Numbers

17-39

Language

English

DOI

10.1016/S0021-9673(00)83016-2

Abstract

Theoretical assumptions concerning the influence of the composition of binary mobile phases on capacity ratios in adsorption chromatography were verified using model experiments with chromatography of four N,N-dimethyl-p-aminobenzeneazobenzoylamides on columns packed with silica (Porasil A) in binary mobile phases composed of ethyl acetate and cyclohexane. In this system, good agreement was found between the experimental values of the elution characteristics (retention volume, peak width, resolution and number of theoretical plates) in isocratic and gradient elution chromatography and the values calculated according to the theoretical relationships derived in Parts I and II of this series. Twenty different gradient functions (linear, convex and concave) were tested and the agreement between experiment and theory was found to be satisfactory in all cases. 1974.