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8722116 
Journal Article 
Standardization of Coscinium fenestratum with reference to berberine by high performance thin layer chromatography 
Chitra, K; Sujatha, K; Polisetti, H; Karri, S; Umamaheswara Reddy, C 
2011 
0 
Research Journal of Pharmaceutical, Biological and Chemical Sciences
ISSN: 0975-8585 
2 
2 
226-229 
English 
A simple and reproducible HPTLC method for the determination of berberine in Coscinium fenestratum was developed and described. The HPTLC method involves separation of components by TLC on precoated silica gel 60 F 254 plate with a solvent system of cyclohexane : chloroform : glacial acetic acid (4.5 : 4.5 : 1) and detection at 366 nm in absorbance mode. The sensitivity of HPTLC method was found to be 0.20μg and the linearity was observed in the range of 0.2 μg to 5.00 μg.The berberine content of 4.67% was observed in the test sample. 2010 RJPBCS. 
Berberine; Coscinium fenestratum; Standardization