Health & Environmental Research Online (HERO)


Print Feedback Export to File
8737041 
Journal Article 
Isolation of soil bacteria for bioremediation of hydrocarbon contamination 
Ilyina, A; Castillo Sanchez, MI; Villarreal Sanchez, JA; Ramirez Esquivel, G; Candelas Ramirez, J 
2003 
1 
Moskovskii Gosudarstvennyi Universitet. Vestnik. Seriya 2: Khimiya
ISSN: 0579-9384 
44 
1 
88-91 
English 
Bacterial strains were isolated from sites impacted by spill of petroleum hydrocarbons for the development a product (COBE-10) applicable in soil bioremediation. Initially, 82 bacterial strains were isolated in selective agars (agar diesel, combustion oil and petroleum). After monitoring absorbance change of mineral media (containing petroleum as sole carbon source) inoculated by isolated strains, 30 strains were selected. The strains were evaluated for their potential to degrade the hydrocarbons of petroleum in soil, artificially contaminated under laboratory conditions. Based on DTPH/day, finally 6 strains were selected. Seven carrier materials were tested to select suitable vehicle for final formulation of COBE-10. Ex -situ evaluation of the developed product (COBE-10) was performed in field with soils contaminated with diesel and refinery wastes. The field test showed a high efficiency of biopreparation COBE-10.