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8745907 
Journal Article 
Production of Next-Generation Biodiesel from High Yielding Strains of Microorganisms: Recent Advances 
Adetunji, CO; Olaniyan, OT; Okeke, NE 
2020 
Springer Science and Business Media B.V. 
Nanotechnology in the Life Sciences 
31-43 
English 
It has been discovered that the production of cost-effective biofuels most especially from renewable material is the only dependable solution that could resolve climate change, transportation sector, and energy security. There are several disadvantages alluded to the utilization of petroleum diesel which include the high cost of production and the various hazards which affect our healthy environment and human health. The discovery and the production of fatty acid methyl esters microorganisms that are oleaginous in nature have been discovered as the only sustainable solution that could serve as a permanent replacement to all the adverse effects and challenges encountered during the production of petroleum. This chapter intends to provide comprehensive details about the recent trends on the production of biodiesel as a safe, eco-friendly, economical, and sustainable biofuel. Moreover, the application of biotechnology such as genetic engineering, strain improvement, recent advancement in fermentation, optimization, and production were discussed in detail. Some recent strategies used in structural biology that could led to increase in the yield of biodiesel were discussed in detail such as synthetic scaffolds, knockout/knockdown of competitive pathways, types of plasmids and its copy numbers, enhancement of ribosome binding sites, promoter engineering, modification of key enzymes, directed evolution, and codon optimization. Also, we discussed extensively on the current trends and principle that regulates the metabolomics engineering of diverse microorganisms that have the potential to produce biodiesel. © Springer Nature Switzerland AG 2020. 
Biodiesels; Eco-friendly; Enzymes; Fatty acid methyl esters; Metabolic pathways; Microorganisms; Oleaginous microbes; Promoter engineering; Sustainable biofuel; Transesterification