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8753065 
Journal Article 
Effects of local microbial bioaugmentation and biostimulation on the bioremediation of total petroleum hydrocarbons (TPH) in crude oil contaminated soil based on laboratory and field observations 
Suja, F; Rahim, F; Taha, MR; Hambali, N; Rizal Razali, M; Khalid, A; Hamzah, A 
2014 
1 
International Biodeterioration & Biodegradation
ISSN: 0964-8305
EISSN: 1879-0208 
90 
115-122 
English 
WOS:000335872000017 
This study investigated factors enhancing the performance of the bioremediation of Total Petroleum Hydrocarbons (TPHs) in crude oil-contaminated soil in laboratory and field observations. The bioaugmentation process used local microbial consortia (MC1, MC2 and MC3) combined with the biostimulation processes of nutrient addition (mineral-salt medium, MSM and NPK) and enhanced air stimulation (air supply and Oxygen Releasing Compound (ORC™)). The microcosm tests were conducted in tank and soil column setups, whereas the field test was performed in test plots inside an oil and gas facility in Malaysia. In the microcosm tank experiment, the combination of bioaugmentation (10% inoculum size of MC3) and MSM biostimulation yielded the highest TPH degradation of 79% of the total. In the column experiments, the degradation of TPHs in the top soil was highest in columns combining bioaugmentation and nutrient addition, whereas in the bottom soil, the degradation of TPHs was highest in columns combining bioaugmentation with the addition of both nutrients and ORCs. In the field demonstration, 97% of the TPHs were degraded in the top soil (0-1m) when bioaugmented with MC2. The kinetic analysis study of the microcosm tank showed that a combination of both biostimulation and bioaugmentation in the soil column achieved the fastest rate constant of 0.0390day-1. The field test also demonstrated a comparable rate constant of 0.0339day-1. The kinetic rate constants in both the laboratory and field indicated that the best treatment method for the contaminated site is a combination of MC3 bioaugmentation and nutrient biostimulation. © 2014 Elsevier Ltd. 
Bioaugmentation; Bioremediation; Biostimulation; Biotechnology; Total petroleum hydrocarbon