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8755112 
Journal Article 
Ex-situ biodegradation of petroleum hydrocarbons using Alcanivorax borkumensis enzymes 
Kadri, T; Magdouli, S; Rouissi, T; Brar, SK 
2018 
Yes 
Biochemical Engineering Journal
ISSN: 1369-703X 
132 
279-287 
English 
WOS:000433266500031 
Bioremediation for degradation of hydrocarbons is a widely used alternative for the recovery of contaminated sites. The current study aimed to use Alcanivorax borkumensis crude enzyme preparation as an agent for enhanced microbial hydrocarbons biodegradation in contaminated water and soil. The inoculum and hydrocarbons concentration have a remarkable effect on the biodegradation with the crude enzymes. The high enzymatic production reaching 145.71 U/mg for alkane hydroxylase, 3628.57 U/mg for lipase and 2200 U/mg for esterase led to a significant degradation efficiency of the different concentrations of petroleum hydrocarbon substrates reaching 73.75% for 5000 ppm of hexadecane, 82.80% for 1000 ppm of motor oil, 64.70% for 70 ppm of BTEX and 88.52% for 6000 ppm of contaminated soil. The study suggested that Alcanivorax borkumensis is a potential hydrocarbon-degrading bacterium with higher enzymatic capacities for bioremediation of hydrocarbon-polluted environment. © 2018 
Alcanivorax borkumensis; Biodegradation; Enzymes production; Petroleum hydrocarbons