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9088407 
Journal Article 
Fluorometric measurement of tyrosine in serum and plasma 
Ambrose, JA 
1974 
Yes 
Clinical Chemistry
ISSN: 0009-9147
EISSN: 1530-8561 
AMER ASSOC CLINICAL CHEMISTRY 
WASHINGTON 
20 
505-510 
English 
This fluorometric procedure for determining tyrosine is that of Ambrose et al., and is based on the fluorometric method of Waalkes and Udenfriend. Studies of the latter procedure and of the various published colorimetric procedures for tyrosine led to a simplified procedure in which the extraction step involving ethylene dichloride was eliminated without loss of specificity. In addition, the instability of the reaction mixture containing nitrosonaphthol, nitric acid, and sodium nitrite encountered in previous fluorometric methods has been corrected by use of a phosphoric acid reagent.