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HERO ID
9088407
Reference Type
Journal Article
Title
Fluorometric measurement of tyrosine in serum and plasma
Author(s)
Ambrose, JA
Year
1974
Is Peer Reviewed?
Yes
Journal
Clinical Chemistry
ISSN:
0009-9147
EISSN:
1530-8561
Publisher
AMER ASSOC CLINICAL CHEMISTRY
Location
WASHINGTON
Volume
20
Issue
4
Page Numbers
505-510
Language
English
PMID
4818207
DOI
10.1093/clinchem/20.4.505
Web of Science Id
WOS:A1974S573000022
Abstract
This fluorometric procedure for determining tyrosine is that of Ambrose et al., and is based on the fluorometric method of Waalkes and Udenfriend. Studies of the latter procedure and of the various published colorimetric procedures for tyrosine led to a simplified procedure in which the extraction step involving ethylene dichloride was eliminated without loss of specificity. In addition, the instability of the reaction mixture containing nitrosonaphthol, nitric acid, and sodium nitrite encountered in previous fluorometric methods has been corrected by use of a phosphoric acid reagent.
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