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9209312 
Journal Article 
Bradykinin parallels thyrotropin-releasing hormone actions on prolactin release from rat anterior pituitary cells 
Drouhault, R; Abrous, N; David, JP; Dufy, B; , 
1987 
1 
Neuroendocrinology
ISSN: 0028-3835
EISSN: 1423-0194 
KARGER 
BASEL 
360-364 
English 
3118231 
WOS:A1987K163200013 
Bradykinin (BK), a nonapeptide, originally discovered in blood, is also present in neurons and fibers of the hypothalamus. We tested the putative releasing factor properties of BK on prolactin (PRL) release from anterior pituitary cells in vitro. BK stimulated the release of PRL in a dose-dependent manner, the threshold concentration being in the range. 0.1-1.0 nM. The release of PRL induced by BK at 1 nM concentration was about 2-fold, delayed and sustained over many minutes. Higher concentrations of BK stimulated PRL release in two phases. The shape of the BK-induced PRL release was superficially similar to that induced by thyrotropin-releasing hormone (TRH). 10 nM BK and 10 nM TRH induced about a 4-fold increase in PRL release within 5 min, followed by a gradual recovery to basal secretion. These results indicate that this peptide can act directly at the anterior pituitary gland to release PRL. Phorbol ester also promoted PRL release over the range of 1-10 nM, but the time course of the release was somewhat different.