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92359 
Journal Article 
Nutrient limitation of epilithic and epixylic biofilms in ten North American streams 
Tank, JL; Dodds, WK 
2003 
Yes 
Freshwater Biology
ISSN: 0046-5070
EISSN: 1365-2427 
BLACKWELL PUBLISHING LTD 
OXFORD 
48 
6 
1031-1049 
WOS:000182948500007 
1. Nutrient diffusing substrata were used to determine the effect of added inorganic nitrogen (N) and phosphorus (P) on the development of epilithic and epixylic biofilms in 10 North American streams. Four treatments of diffusing substrata were used: Control (agar only), N addition (0.5 m NaNO3 ), P addition (0.5 m KH2 PO4 ), and N + P combined (0.5 m NaNO3 + 0.5 m KH2 PO4 ). Agar surfaces were covered with glass fibre filters (for epilithon) or discs of untreated white oak wood veneer (for epixylon).



2. We found that if algae showed significant response to nutrient addition, N limitation (either N alone or N with P) was the most frequent response both on GF/F filters and on wood. Despite the low dissolved nutrient concentrations in our study streams, more than a third of the streams did not show any response to N or P addition. In fact, P was never the sole limiting nutrient for algal biofilms in this study.



3. Nutrient addition influenced algal colonisation of inorganic versus organic substrata in different ways. The presence of other biofilm constituents (e.g. fungi or bacteria) may influence whether algal biomass on wood increased in response to nutrient addition. Algae on organic and inorganic substrata responded similarly to nutrient addition in only one stream.



4. Fungal biomass on wood was nutrient limited in six of 10 study streams. N limitation of fungal biomass (with or without secondary P limitation) was most frequent, but P limitation did occur in two streams.



5. Our results show that biomass responses to nutrient addition by the heterotrophic and autotrophic components of the epixylic biofilm were different, though both experienced the same stream nutrient conditions. For algae and fungi growing on wood, limiting nutrients were rarely similar. Only three of nine streams showed the same biomass response to nutrient addition, including two that showed no significant change in biomass despite added nutrients. 
biofilm; epilithon; fungi; nitrogen; nutrients; periphyton; phosphorus; stream