Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
932588
Reference Type
Journal Article
Title
Clean recovery of antioxidant flavonoids from citrus peel: Optimizing an aqueous ultrasound-assisted extraction method
Author(s)
Londoño-Londoño, J; Lima, VRd; Lara, O; Gil, A; Pasa, TBC; Arango, GJ; Pineda, JRR
Year
2010
Is Peer Reviewed?
Yes
Journal
Food Chemistry
ISSN:
0308-8146
EISSN:
1873-7072
Publisher
Elsevier
Volume
119
Issue
1
Page Numbers
81-87
DOI
10.1016/j.foodchem.2009.05.075
Web of Science Id
WOS:000271818200013
URL
http://linkinghub.elsevier.com/retrieve/pii/S0308814609007833
Exit
Abstract
One of the main reasons for unsuccessful recovery of flavonoids from citrus by-products is the absence of effective extraction procedures. In this work, flavonoid fractions were obtained from citrus peels (lime, orange and tangerine) growing in South American cultivars using an optimized aqueous ultrasound-assisted extraction method with high yield (40.25 +/- 12.09 mg of flavonoid fraction/g peel). Total phenolic content in flavonoid fractions obtained from different sources was 74.80 +/- 1.90, 66.36 +/- 0.75 and 58.68 +/- 4.01 mg GAE (gallic acid equivalents)/g, for lime, orange and tangerine, respectively. The composition of flavonoid fractions was established by using HPLC/MS. Orange peel contained hesperidin, neohesperidin, diosmin, nobiletin and tangeritin, being the most complex source. Tangerine peel was the simplest source and contained only hesperidin and neohesperidin. Using the thiobarbituric acid-reactive substances (TBARS) assay it was demonstrated that all flavonoid fractions were able to inhibit cupper (Cu super(2+)) or peroxynitrite (ONOO super(-)) induced human low density lipoprotein (LDL) oxidation. Differences in the antioxidant activity of individual components from flavonoid fractions were also observed.
Keywords
Citrus peel; Ultrasound-assisted extraction; Flavonoids; LDL; HPLC/MS
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity