Abdel-Wahhab, MA; El-Nekeety, AA; Hathout, AS; Salman, AS; Abdel-Aziem, SH; Sabry, BA; Hassan, NS; Abdel-Aziz, MS; Aly, SE; Jaswir, I
This study aimed to identify the bioactive compounds of the ethyl acetate extract of Aspergillus niger SH2-EGY using GC-MS and to evaluate their protective role against aflatoxin B1 (AFB1)-induced oxidative stress, genotoxicity and cytotoxicity in rats. Six groups of male Sprague-Dawley rats were treated orally for 4 weeks included the control group, AFB1-treated group (80 μg/kg b.w); fungal extract (FE)-treated groups at low (140) or high dose (280) mg/kg b.w and the groups treated with AFB1 plus FE at the two tested doses. The GC-MS analysis identified 26 compounds. The major compounds found were 1,2,3,4,6-Penta-trimethylsilyl Glucopyranose, Fmoc-L-3-(2-Naphthyl)-alanine, D-(-)-Fructopyranose, pentakis (trimethylsilyl) ether, bis (2-ethylhexyl) phthalate, trimethylsilyl ether-glucitol, and octadecanamide, N-(2- methylpropyl)-N-nitroso. The in vivo results showed that AFB1 significantly increased serum ALT, AST, creatinine, uric acid, urea, cholesterol, triglycerides, LDL, carcinoembryonic antigen, alpha-fetoprotein, interleukin-6, Malondialdehyde, nitric oxide, Bax, caspase-3 and P53 mRNA expression, chromosomal aberrations and DNA fragmentation. It decreased serum TP, albumin, HDL, Bcl-2 mRNA expression, hepatic and renal TAC, SOD and GPx content and induced histological changes in the liver and kidney. FE prevented these disturbances in a dosage-dependent manner. It could be concluded that A. niger SH2-EGY extract is safe a promising agent for pharmaceutical and food industries.
Aflatoxin B1; Aspergillus niger; Bioactive compounds; Cytotoxicity; Genotoxicity; Oxidative stress; 1,2,3,4,6 pentatrimethylsilyl glucopyranose; aflatoxin B1; alanine aminotransferase; alpha fetoprotein; antioxidant; aspartate aminotransferase; Aspergillus niger extract; bis (2 ethylhexyl)phthalate; carcinoembryonic antigen; caspase 3; cholesterol; creatinine; dextro fructopyranose; Fmoc levo 3 (2 naphthyl)alanine; fungal extract; interleukin 6; low density lipoprotein; malonaldehyde; messenger RNA; n (2 methylpropyl) n nitroso; nitric oxide; octadecanamide; pentakis (trimethylsilyl)ether; protein Bax; protein p53; triacylglycerol; trimethylsilyl ether glucitol; unclassified drug; unindexed drug; urea; uric acid; aflatoxin B1; antioxidant; alanine aminotransferase blood level; alpha fetoprotein blood level; animal cell; animal experiment; animal model; animal tissue; antioxidant activity; Article; aspartate aminotransferase blood level; Aspergillus niger; cell mediated cytotoxicity; cell protection; cholesterol blood level; chromosome aberration; controlled study; creatinine blood level; DNA fragmentation; dose response; drug megadose; drug screening; genotoxicity; in vivo study; low density lipoprotein cholesterol level; low drug dose; male; mass fragmentography; nonhuman; oxidative stress; priority journal; rat; triacylglycerol blood level; urea blood level; uric acid blood level; animal; Aspergillus niger; drug effect; drug inactivation; Sprague Dawley rat; Aflatoxin B1; Animals; Antioxidants; Aspergillus niger; DNA Fragmentation; Inactivation, Metabolic; Male; Oxidative Stress; Rats; Rats, Sprague-Dawley