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9427321 
Journal Article 
Chemical constituents and cytotoxic activity of cassia glauca lan. leaves 
El-Sayed, MM; Abdel-Aziz, MM; Abdel-Gawad, MM; Abdel-Hameed, ESS; Ahmed, WS; Abdel-Lateef, EE 
2013 
1 
Life Science Journal
ISSN: 1097-8135 
10 
3 
1617-1625 
English 
The defatted 85 % methanolic extract from the leaves of Cassia gluaca Lan. (Family Leguminaceae) showed high cytotoxic effect against liver carcinoma cell line (HepG2) (IC50 = 17 μg /ml). Therefore this extract was fractionated using different organic solvents; chloroform, ethyl acetate and n-butanol. Each fraction was submitted to chromatographic separation and the structure of the isolated compounds were elucidated using physical properties and certain spectroscopic analysis. From the chloroform fraction, three compounds; Di-(2-ethylhexyl) phthalate (DEHP) (1), apigenin (2) and luteolin (3) were isolated. Quercetin (4), quercetin-3-O-β-D-glucopyranoside (5) and kaempferol-3-O-rutinoside (6) were isolated and identified from ethyl acetate fraction whereas from the nbutanol fraction three compounds were identified as D (+)-pinitol (7), quercetin-3-O-α-L-rhamnopyranosyl-(1→6)- β-D-glucopyranoside (Rutin) (8) and quercetin-3-O-[α-L-rhamnopyronosyl-(1→2)-α-L-rhamnopyro nosyl-(1→6)-]- β-D-glucopyronoside (9). The cytotoxicity of the isolated compounds 5, 6 and 9 was evaluated against HepG2 cell lines. The results showed that the three compounds 5, 6 and 9 have promising cytotoxicity with IC50 = 16.1, 14.3 and 15.2 μg/ml respectively. From the above results and our previous reports, the methanol extract of the leaves of Cassia glauca could be used as promising antioxidant and anticancer agent after more in vitro and in vivo studies. 
Cassia gluaca; Flavonoids; Liver carcinoma