Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
9427321
Reference Type
Journal Article
Title
Chemical constituents and cytotoxic activity of cassia glauca lan. leaves
Author(s)
El-Sayed, MM; Abdel-Aziz, MM; Abdel-Gawad, MM; Abdel-Hameed, ESS; Ahmed, WS; Abdel-Lateef, EE
Year
2013
Is Peer Reviewed?
1
Journal
Life Science Journal
ISSN:
1097-8135
Volume
10
Issue
3
Page Numbers
1617-1625
Language
English
URL
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84884128547&partnerID=40&md5=8ec5920cb435d766bcffcf0b921137fa
Exit
Abstract
The defatted 85 % methanolic extract from the leaves of Cassia gluaca Lan. (Family Leguminaceae) showed high cytotoxic effect against liver carcinoma cell line (HepG2) (IC50 = 17 μg /ml). Therefore this extract was fractionated using different organic solvents; chloroform, ethyl acetate and n-butanol. Each fraction was submitted to chromatographic separation and the structure of the isolated compounds were elucidated using physical properties and certain spectroscopic analysis. From the chloroform fraction, three compounds; Di-(2-ethylhexyl) phthalate (DEHP) (1), apigenin (2) and luteolin (3) were isolated. Quercetin (4), quercetin-3-O-β-D-glucopyranoside (5) and kaempferol-3-O-rutinoside (6) were isolated and identified from ethyl acetate fraction whereas from the nbutanol fraction three compounds were identified as D (+)-pinitol (7), quercetin-3-O-α-L-rhamnopyranosyl-(1â6)- β-D-glucopyranoside (Rutin) (8) and quercetin-3-O-[α-L-rhamnopyronosyl-(1â2)-α-L-rhamnopyro nosyl-(1â6)-]- β-D-glucopyronoside (9). The cytotoxicity of the isolated compounds 5, 6 and 9 was evaluated against HepG2 cell lines. The results showed that the three compounds 5, 6 and 9 have promising cytotoxicity with IC50 = 16.1, 14.3 and 15.2 μg/ml respectively. From the above results and our previous reports, the methanol extract of the leaves of Cassia glauca could be used as promising antioxidant and anticancer agent after more in vitro and in vivo studies.
Keywords
Cassia gluaca; Flavonoids; Liver carcinoma
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity