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9429149 
Journal Article 
Determination of phthalates in leachates from commonly used plastic pouches and in biological tissues of leachates exposed rats 
Ojha, P; Ojha, CS; Sharma, VP 
2005 
Chk 
Indian Journal of Environmental Protection
ISSN: 0253-7141 
Kalpana Corporation 
25 
11 
1025-1028 
English 
Phthalates are widely used as plasticizers to impart softness and flexibility to the finished plastic and constitute 40-60% of the polymer. In present study, attempts were made to estimate phthalates content in leachates from commonly used plastic (pouches) in simulating condition media, such as water and normal saline using high pressure liquid chromatography (HPLC). The phthalates measured were di-ethyl-hexyl phthalates (DEHP), di-butyl-phthalate (DBP) and di-methyl-phthalate (DMP). In order to assess the continuous leachability, if any, the procedure of exraction was repeated 3 times in identical conditions using same plastic and no significant leachability of any of the phthalate could be observed in 2nd, 3rd and 4th extractions revealing no leachability of phthalates on repeated extraction. In order to assess the accumulation of phthalates in experimental rats fed with leachates for a period of 90 day. The maximum accumulation was observed in liver of rats fed with leachates as compare to vehicle fed animals. Since phathalates are known hepatotoxic and also known to have estrogenic potential. The present study on leachability and accumulation in vital organ may be of relevance in correlating the toxic effect of plastic leachates. © 2005 - Kalpana Corporation. 
High pressure liquid chromatography (HPLC); Leachates; Plastics; Animalia