US EPA

95118 

Journal Article 

Reversed-phase high-performance liquid chromatographic separation of human phenolic metabolites of propoxur (Baygon), carbofuran and carbaryl 

Keiser, JE; Kirby, KW; Tremmel, F 

1983 

Yes 

Journal of Chromatography A
ISSN: 0021-9673
EISSN: 1873-3778 

ELSEVIER SCIENCE BV 

AMSTERDAM 

259 

1 

186-188 

English 

6406526 

10.1016/S0021-9673(01)87995-4 

WOS:A1983QK06800022 

High performance liquid chromatography (HPLC) separation of human phenolic metabolites of the carbamate (302114) insecticides propoxur (114261), carbofuran (1563662), and carbaryl (63252) were investigated. The phenols examined were 2,3-dihydro-2,2-dimethyl-3-keto-7-hydroxybenzofuran, 2,3-dihydro-2,2-dimethyl-7-hydroxybenzofuran, 2-isopropoxyphenol (4812208), and 1-naphthol (90153). HPLC was performed on a reversed phase column. Solvent mixtures were tested. Two different gradient elutions using an acetonitrile/water solvent mixture were compared: 0 to 60 percent (10 minutes) and 30 to 60 percent (10 minutes). Several solvent mixtures were capable of separating the four phenolic metabolites on the reversed phase column including: 35 to 65 acetonitrile/water; 55 to 45 methanol/water; and 35 to 65 tetrahydrofuran/water. Peak 1 in the 0 to 60 percent, 10 minute, gradient was less efficient than the 30 to 60 percent gradient. With the 30 to 60 percent gradient the peaks also eluted in less than 4 minutes as compared to a 5 minute period in the 0 to 60 percent gradient. The authors conclude that the HPLC procedures enable separation of human urinary metabolites of three commonly used carbamate insecticides. 

1 naphthol; carbaril; carbofuran; phenol; propoxur; 2,3 dihydro 7 hydroxy 2,2 dimethyl 3 benzofuranone; 2,3 dihydro 7 hydroxy 2,2 dimethylbenzofuran; carbamyl; drug analysis; drug determination; drug identification; drug metabolism; drug urine level; high performance liquid chromatography; human; isopropoxyphenol; methodology; reversed phase liquid chromatography; Carbaryl; Carbofuran; Chromatography, High Pressure Liquid; Human; Insecticides, Carbamate; Phenols; Propoxur