US EPA

9574592 

Journal Article 

[Effect of nandrolone phenylpropionate on expression of hepatic albumin-mRNA and androgen receptor in burned rats] 

Cen, Y; Li, K; Liu, XX 

2003 

1 

Zhongguo Xiufu Chongjian Waike Zazhi
ISSN: 1002-1892 

17 

6 

439-441 

Chinese 

14663936 

OBJECTIVE: To study the mechanism of nandrolone phenylpropionate (NP) on hepatic albumin mRNA and androgen receptor(AR) in burned rats.

METHODS: Thirty-two Wistar rats with a deep second-degree cutaneous burn of 20% total body surface area were randomly divided into two groups: NP group (experimental group, 5 mg/kg NP) and normal saline as control group every other day. The expression copy quantity of albumin-mRNA and mean integrated absorbency(mIA) of AR in liver tissue were measured by quantitative fluorescent RT-PCR and immunohistochemistry respectively on the 4th, 7th, 14th and 21st days of post-burned.

RESULTS: The expression levels of albumin-mRNA and AR in liver tissue in NP group were much higher than those in control group. The albumin-mRNA and AR expression increased significantly (P < 0.05) after 7 and 14 days, while the expression had no significant difference between NP group and control group on the 4th day. A positive correlation occurred between the expression level of albumin-mRNA and the quantity of AR in liver tissue(r = 0.936, P < 0.05).

CONCLUSION: Nandrolone phenylpropionate up-regulated respectively the expression of albumin-mRNA and the density of AR in liver tissue.