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975860 
Journal Article 
Abstract 
The aerobic one-electron oxidation of ferrocytochrome C by peroxynitrite is an oxygen-dependent reaction that does not involve carbonate radical 
Peterson, J; Lopez-Manzano, E; Pearce, LL 
2008 
Yes 
Free Radical Biology and Medicine
ISSN: 0891-5849
EISSN: 1873-4596 
45 
Suppl. 
S62-S62 
English 
WOS:000260867900171 
is part of a larger document 3452652 SFRBM's 15th Annual Meeting: Program and Abstracts
The mitochondrial electron transport chain (ETC) can readily undergo redox reactions with a variety of organic and inorganic species, including peroxynitrite, preferentially a two-electron oxidant. There is a good deal of evidence supporting the interaction of peroxynitrite with carbon dioxide in physiological fluids, leading to the generation of carbonate radical, a one-electron oxidant. Therefore, the question of whether peroxynitrite appears to act as a one- or two-electron oxidant towards metalloenzymes, like those of the ETC, is of some interest. We find that peroxynitrite appears to behave as a one-electron oxidant towards ferrocytochrome c, but the reaction seems to be independent of carbon dioxide. This is in good agreement with the earlier findings of others [Arch. Biochem. Biophys. (1996) 333, 49-58]. However, more surprisingly, the reaction shows a clear dependence on the presence of oxygen. in contrast, the reaction of peroxynitrite with ferrocyanide, also a one-electron process, is both oxygen and carbon dioxide independent. the mechanistic role of oxygen in one-electron oxidations by peroxynitrite is currently under further investigation. 
Society for Free Radical Biology and Medicine 15th Annual Meeting 
Indianapolis, IN 
November 19-23, 2008