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975861 
Journal Article 
Abstract 
Combined effects of nitric oxide and hypoxia on breast cancer cell migration 
Hickok, JR; Sahni, S; Krock, KM; Thomas, DD 
2008 
Yes 
Free Radical Biology and Medicine
ISSN: 0891-5849
EISSN: 1873-4596 
45 
Suppl. 
S53-S53 
English 
WOS:000260867900144 
is part of a larger document 3452652 SFRBM's 15th Annual Meeting: Program and Abstracts
Tumor cell invasion is a major component of breast cancer aggressiveness and patient mortality. Although there are a variety of factors that contribute to invasive phenotypes, nitric oxide (NO) plays an important role in influencing the metastatic process. NO can alter tumor cell migration and invasion in either a positive or negative fashion. as these differences may simply be a function of the concentration of NO, we set out to investigate the concentration-dependent parameters of NO that differentially regulate tumor cell migration. We correlate the degree of tumor cell (MDA-MD-231) migration with the concentration and duration of NO exposure. Furthermore, we demonstrate that hypoxia (1% O2) influences the migratory potential of tumor cells by altering the steady-state concentrations of NO. Finally, using both chemical (NO donors) and biological sources (RAW 274.1 cells), we found that both the rate of NO production and its rate of metabolism are critical determinants of tumor cell migration. 
Society for Free Radical Biology and Medicine 15th Annual Meeting 
Indianapolis, IN 
November 19-23, 2008