Health & Environmental Research Online (HERO)


Print Feedback Export to File
975870 
Journal Article 
Abstract 
The role of nitric oxide in the tolerance induction to LPS 
Melo, ES; Barbeiro, DF; Moretti, AIS; Soriano, FG 
2008 
Yes 
Free Radical Biology and Medicine
ISSN: 0891-5849
EISSN: 1873-4596 
45 
Suppl. 
S136-S136 
English 
WOS:000260867900385 
is part of a larger document 3452652 SFRBM's 15th Annual Meeting: Program and Abstracts
The nitric oxide has participation in the tolerance to the LPS “in vitro” and intervenes with the development of the tolerance in the macrophages, regulating the production of cytokines. This study investigated the nitric oxide role in the production of the cytokines from the macrophages in the model of tolerance to the LPS. Peritoneal macrophages were collected from nitric oxide synthase (iNOS) knock out mice (KO) or wild type (C57). Cell culture with 106 cells/ml in medium RPMI were divided in three groups: CGcontrol group, GL- stimulation with LPS (10 μg/ml) in the last 24 hours of culture, GT- stimulation with LPS (10 μg/ml) in the last 48 hours of culture. the supernatants were collected after 72 hours and analyzed by ELISA. 
Society for Free Radical Biology and Medicine 15th Annual Meeting 
Indianapolis, IN 
November 19-23, 2008