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9779638 
Journal Article 
The binding of thyroid hormones to bovine serum albumin as measured by circular dichroism 
Okabe, N; Manabe, N; Tokuoka, R; Tomita, K 
1975 
Yes 
Journal of Biochemistry
ISSN: 0021-924X
EISSN: 1756-2651 
77 
1? 
181-186 
English 
1169236 
The circular dichroism (CD) spectra of 3,5,3'-triiodothyronine-bovine serum albumin (T3-BSA) and thyroxine-bovine serum albumin (T4-BSA) complexes were measured at the various ratios of T3 or T4 to BSA (T/P) in the wavelength region of 200-350 nm. No spectral change was observed in the chromophoric wavelength region of 250-350 nm. The value of [omicron] at 319 nm of the induced Cotton effect increased with increase of the T/P ratio in both complexes, and in T4-BSA complex, it increased remarkably. The number of thyroid hormone molecules bound to one BSA molecule was estimated to be one in the case of T4-BSA complex and four to five in T3-BSA.