US EPA

979787 

Journal Article 

Dual Effects of Interaction Between Meloxicam, Diclofenac Sodium or Tramadol and Nitrogen Species Radicals: In vitro Comparative Study 

Al-Nimer, MSM; Ali, EA 

2009 

Yes 

International Journal of Pharmacology
ISSN: 1811-7775 

5 

1 

86-89 

English 

WOS:000266812100013 

This study aimed to investigate the interaction between synthetic peroxynitrite or sodium nitroprusside (nitric oxide donor) and meloxicam, diclofenac sodium or tramadol HCl. Meloxicam, diclofenac sodium or tramadol HCl (100-500 mu g) were incubated in phosphate buffer saline in presence or absence of synthetic peroxynitrite (180 mu M) or sodium nitroprusside as nitric oxide donor (10 mM). The level of peroxynitrite and nitric acid in solution were measured using UV-visible spectrophotometer. The results showed that meloxicam scavenged synthetic peroxynitrite and involved in peroxynitrite mediated phenol nitration when it incubated alone in phosphate buffer. All tested compounds, in vitro, behaved like sodium nitroprusside in releasing nitric oxide. Both meloxicam and diclofenac sodium reduced the activity of sodium nitroprusside-releasing nitric oxide. Tramadol HCl was not interacted with sodium nitroprusside at any concentration. We concluded that selective or non selective nonsteroidal anti-inflammatory drugs reduced the activity of nitric oxide donor while tramadol HCl is free from this effect. 

Meloxicam; diclofenac sodium; tramadol; nitric oxide; peroxynitrite