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9833473 
Journal Article 
Simultaneous determination of cysteine, cystine and 18 other amino acids in various matrices by high-performance liquid chromatography 
Campanella, L; Crescentini, G; Avino, P 
1999 
Yes 
Journal of Chromatography A
ISSN: 0021-9673
EISSN: 1873-3778 
833 
2 
137-145 
English 
10081828 
An extraction protocol and a reversed-phase high-performance liquid chromatographic method for the simultaneous determination of cysteine, cystine and 18 other amino acids in biological and vegetable samples are described. Among the different methods proposed for amino acid determination, phenylisothiocyanate was used as the reagent for derivatization. Chromatograms obtained in the analysis of standard solutions and actual samples are reported, together with regression equation, correlation coefficient (> 0.999 for all), limit of detection and recoveries (between 86 and 102% for all the examined matrices) for each amino acid. Practical protocol and method applications in normal patients and patients affected by different pathologies, and in algal products are discussed.