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1022831 
Journal Article 
Free vitamin D3 metabolites in Cestrum diurnum leaves 
Prema, TP; Raghuramulu, N 
1994 
1 
Phytochemistry
ISSN: 0031-9422
EISSN: 1873-3700 
BIOSIS/95/01270 
OXFORD 
OXFORD 
677-681 
English 
BIOSIS COPYRIGHT: BIOL ABS. The occurrence of free and glycosidic vitamin D3 and its metabolites in Cestrum diurnum leaves was investigated. The chloroform extract of the leaves (containing free vitamin D3 and its metabolites) and the chloroform-methanol (1:2) extract (containing glycosidic vitamin D3 and its metabolites) of the residue after glycosidase treatment were partially purified by column chromatography. Fractions corresponding to authentic vitamin D3, 25-hydroxy vitamin D3 (25-OH-D3) and 1,25-dihydroxy vitamin D3 (1,25-(OH)2D3) were found to be biologically active, These fractions were also analysed and quantified by HPLC. This is the first demonstration of the presence of free vitamin D3, 25-OH-D3 and 1,25-(OH)2D3 in a calcinogenic plant. Apart from the free forms, the corresponding glycosidic metabolites were also found. The concentration of free metabolites was much higher than that of the glycosidic forms. 
Biochemical Studies-Vitamins; Biophysics-General Biophysical Techniques; Nutrition-Fat-Soluble Vitamins; Toxicology-Veterinary Toxicology; Plant Physiology; Plant Physiology; Pharmacognosy and Pharmaceutical Botany; Solanaceae 
• Chloroform Combined (current)
     Chloroform (original)
• Methanol (Non-Cancer)
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