US EPA

1043382 

Journal Article 

Supercritical fluid extraction of phenol compounds from olive leaves 

Le Floch, F; Tena, MT; Rı́os, A; Valcárcel, M 

1998 

1 

Talanta
ISSN: 0039-9140
EISSN: 1873-3573 

Elsevier B.V. 

46 

5 

1123-1130 

English 

18967237 

10.1016/S0039-9140(97)00375-5 

WOS:000074887400035 

A clean, highly selective supercritical fluid extraction (SFE) method for the isolation of phenols from olive leaf samples was examined. Total phenol extracts were determined using the Folin-Ciocalteu reagent. Dried, ground, sieved olive leaf samples (30 mg) are subjected to SFE, using carbon dioxide modified with 10% methanol at 334 bar, 100 degrees C (CO(2) density 0.70 g ml(-1)) at a liquid flow-rate of 2 ml min(-1) for 140 min. Diatomaceous earth is used to reduce the void volume of the extraction vessel. The influence of extraction variables such as modifier content, pressure, temperature, flow-rate, extraction time, and collection/elution variables, were studied. Supercritical fluid extracts were screened for acid compounds such as carboxylic acids and phenols using Electrospray-MS (in the negative ionization mode). SFE was found to produce higher phenol recoveries than sonication in liquid solvents such as n-hexane, diethyl ether and ethyl acetate. However, the extraction yield obtained was only 45%, using liquid methanol. 

supercritical fluid extraction; phenol; olive leaves