US EPA

1045247 

Journal Article 

Care regimen and lens material influence on silicone hydrogel contact lens deposition 

Zhao, Z; Carnt, NA; Aliwarga, Y; Wei, X; Naduvilath, T; Garrett, Q; Korth, J; Willcox, MD 

2009 

1 

Optometry and Vision Science
ISSN: 1040-5488
EISSN: 1538-9235 

86 

3 

251-259 

English 

19165125 

10.1097/OPX.0b013e318196a74b 

WOS:000264004400011 

PURPOSE: To quantitatively detect proteins and cholesterol extracted from worn silicone hydrogel contact lenses and determine the effect of various lens care solutions on deposit accumulation.

METHODS: Contact lenses, made from different polymers and worn on a daily wear schedule with different lens care solutions, were collected. Lipid and protein deposits were extracted by methanol:chloroform (1:1, v/v) and protein extraction solution (containing urea and surfactant), respectively. Lipid extracts were separated and cholesterol quantified using thin layer chromatography. Protein extracts were quantified using standard techniques.

RESULTS: Among all lenses tested, Balafilcon A lenses exhibited greatest extracted cholesterol (4.1 to 8.2 microg/lens) and total protein (5.4 to 23.2 microg/lens). AQuify was the most effective solution in reducing extracted deposits, especially extracted protein, from Balafilcon A lenses. AQuify and Opti-Free RepleniSH solutions were most effective in reducing extracted cholesterol from Senofilcon A and Galyfilcon A lenses, respectively. Use of Opti-Free Express solution resulted in more extracted protein from Lotrafilcon B lenses than use of other solutions. Generally, Lotrafilcon B, Senofilcon A, and Galyfilcon A lenses accumulated relatively low amount of proteins. Lotrafilcon B lenses accumulated the least amount of cholesterol deposit among all lenses tested regardless of solution used.

CONCLUSIONS: Lens polymer (possibly associated with surface characteristics) is a prominent factor affecting lipid and protein accumulation. Within a lens polymer type, lens care solutions exhibit varying effectiveness in reducing protein and lipid accumulation. 

Silicone hydrogel contact lenses; lens care solutions; lipids; proteins; cholesterol