US EPA

1049301 

Journal Article 

Chemical fingerprint of Hoodia species, dietary supplements, and related genera by using HPTLC 

Rumalla, CS; Avula, B; Shukla, YJ; Wang, YH; Pawar, RS; Smillie, TJ; Khan, IA 

2008 

Yes 

Journal of Separation Science
ISSN: 1615-9306
EISSN: 1615-9314 

Journal of separation science 

31 

22 

3959-3964 

English 

19065611 

10.1002/jssc.200800441 

WOS:000262167500019 

A HPTLC method was developed for simple and rapid chemical fingerprint analysis of four Hoodia species, dietary supplements that claim to contain Hoodia gordonii, and plants from genera related to Hoodia. HPTLC was performed on precoated silica 60F(254 )plates with dichloromethane/methanol/water 75:17:2.2 by volume, as mobile phase. Evaluation of the HPTLC plates was done by using the CAMAG DigiStore2 digital system with winCATS software. The authentication of H. gordonii was achieved by comparing the band colors and R(f) values for TLC fingerprints with those of 11 standard compounds including P57. The developed method was successfully applied for the identification of the 11 pregnane glycosides for four different species of Hoodia, 24 related genera and 13 dietary supplements that claim to contain H. gordonii. Different sample matrices were successfully analyzed, providing a wide range of applicability for this method, including gels, capsules, tablets, sprays, teas, snack bars, powders, and juices. The developed method was validated for specificity, stability, repeatability, and robustness. The results of HPTLC method were verified by LC-UV-MS method. 

Dietary supplements; Hoodia gordonii; Hoodia species; HPTLC; Pregnane glycosides