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1054484 
Journal Article 
PREPARATIVE ISOLATION AND PURIFICATION OF FOUR FLAVONOIDS FROM DAPHNE GENKWA SIEB. ET ZUCC. BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY 
Xie, H; Liang, Y; Ito, Y; Wang, X; Chen, R; He, J; Li, H; Zhang, T 
2011 
Yes 
Journal of Liquid Chromatography and Related Technologies
ISSN: 1082-6076
EISSN: 1520-572X 
34 
19 
2360-2372 
English 
22379361 
WOS:000299376000009 
Four flavonoids including luteolin, apigenin, 3'-hydroxygenkwanin and genkwanin were isolated and purified from Daphne genkwa Sieb. et Zucc. by high-speed countercurrent chromatography (HSCCC). Preparative HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (5:7:5:5, v/v) was successfully performed by increasing the flow rate of the mobile phase from 1.2 to 2.0 mL/min after 260 min. In a one-step operation, 150 mg of the extracts of D. genkwa was separated to yield 8 mg of luteolin, 25.8 mg of apigenin, 23.6 mg of 3'-hydroxygenkwanin and 35.3 mg genkwanin with the purities of 91.2, 97.4, 94.3 and 95.8%, respectively, analyzed by HPLC using area normalization method. The chemical structures of the four compounds were identified by HPLC, ESI-MS, and (1)H NMR. 
Daphne genkwa sieb. et Zucc.; flavonoid; high-speed countercurrent chromatography; luteoline; apiganin; 3-hydroxygenkwanin and genkwanin 
IRIS
• Methanol (Non-Cancer)
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