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Citation
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HERO ID
1071546
Reference Type
Journal Article
Title
DNA damage induced by furocoumarin hydroperoxides plus UV (360 nm)
Author(s)
Epe, B; Häring, M; Ramaiah, D; Stopper, H; Abou-Elzahab, MM; Adam, W; Saha-Möller, CR
Year
1993
Is Peer Reviewed?
Yes
Journal
Carcinogenesis
ISSN:
0143-3334
EISSN:
1460-2180
Volume
14
Issue
11
Page Numbers
2271-2276
Language
English
PMID
8242854
Web of Science Id
WOS:A1993MH92000011
Abstract
When irradiated at 360 nm, furocoumarins with a hydroperoxide group in a side chain efficiently give rise to a type of DNA damage that can best be explained by a photo-induced generation of hydroxyl radicals from the excited photosensitizers. The observed DNA damage profiles, i.e. the ratios of single-strand breaks, sites of base loss (AP sites) and base modifications sensitive to formamidopyrimidine--DNA glycosylase (FPG protein) and endonuclease III, are similar to the DNA damage profile produced by hydroxyl radicals generated by ionizing radiation or by xanthine and xanthine oxidase in the presence of Fe(III)--EDTA. No such damage is observed with the corresponding furocoumarin alcohols or in the absence of near-UV radiation. The damage caused by the photo-excited hydroperoxides is not influenced by superoxide dismutase (SOD) or catalase or by D2O as solvent. The presence of t-butanol, however, reduces both the formation of single-strand breaks and of base modifications sensitive to FPG protein. The cytotoxicity caused by one of the hydroperoxides in L5178Y mouse lymphoma cells is found to be dependent on the near-UV irradiation and to be much higher than that of the corresponding alcohol. Therefore the new type of photo-induced damage occurs inside cells. Intercalating photosensitizers with an attached hydroperoxide group might represent a novel and versatile class of DNA damaging agents, e.g. for phototherapy.
Tags
IRIS
•
tert-Butanol
Considered Studies
Electronic Search
Excluded/ Not on Topic
Methodology/Solvent
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