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HERO ID
1083494
Reference Type
Journal Article
Title
Effect of ascorbic acid supplementation during the inhalation exposure of guinea-pigs to industrial dust on bronchoalveolar lavage and pulmonary enzymes
Author(s)
Kováciková, Z; Ginter, E
Year
1995
Is Peer Reviewed?
Yes
Journal
Journal of Applied Toxicology
ISSN:
0260-437X
EISSN:
1099-1263
Volume
15
Issue
4
Page Numbers
321-324
Language
English
PMID
7594202
Web of Science Id
WOS:A1995RM13100012
Abstract
The aim of this study was to investigate the interaction between ascorbic acid (AA) and inhaled particles separated from the dumped waste of a nickel smelter and refinery. Tricoloured male guinea pigs were exposed in an inhalation chamber to 50 mg kg-1 of < 5 microns particles that mainly consisted of metal oxides. Exposure lasted for 4 weeks (5 days per week and 5 h per day). The drinking water of half of the exposed and half of the control groups was supplemented with 1 g l-1 AA. Each group received 0.4 mmol kg-1 AA in their food. Ascorbic acid supplementation increased the pulmonary AA concentration in both exposed and control groups to the same extent, but in bronchoalveolar lavage fluid the increase was higher in control than in exposed guinea pigs. The number of alveolar macrophages was increased by exposure and AA increased the number only in the exposed group; the acid phosphatase activity of the alveolar macrophages was increased only by AA, and more in the exposed than in the control group. Alkaline phosphatase activity in bronchoalveolar lavage fluid was the same in both supplemented groups, but it was enhanced in the exposed group with a low intake of AA. Effects on lactate dehydrogenase were not consistent. Neither exposure nor AA influenced significantly this enzyme in bronchoalveolar lavage fluid, but in the lung both AA and exposure caused an increase in the activity. The levels were similar in the AA-treated control and exposed guinea pigs.
Keywords
INHALATION EXPOSURE; METAL AEROSOL; ASCORBIC ACID; LUNG; BRONCHOALVEOLAR LAVAGE; GUINEA PIG
Tags
IRIS
•
Chromium VI
Considered
Potentially Relevant Supplemental Material
Acute Exposures
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