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1108333 
Journal Article 
PREPARATIVE SEPARATION AND PURIFICATION OF ALKYLAMIDES FROM ZANTHOXYLUM BUNGEANUM MAXIM BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY 
Wang, S; Xie, JC; Yang, W; Sun, BG 
2011 
Yes 
Journal of Liquid Chromatography and Related Technologies
ISSN: 1082-6076
EISSN: 1520-572X 
TAYLOR & FRANCIS INC 
PHILADELPHIA 
34 
20 
2640-2652 
English 
WOS:000299376300013 
High-speed counter-current chromatography (HSCCC) was successfully applied to preparative isolation and purification of alkylamides, the tingling-taste components, from pericarp extract of Zanthoxylum bungeanum Maxim (Dahongpao). With the lower phase of n-hexane-ethyl acetate-methanol-water (7:3:5:5, v/v) eluted at a flow rate of 1.5mL×min -1, and the revolution speed at 850rpm, 0.19g of alkylamides in purity of 98.0% by the relative peak area of HPLC was obtained from 1.0g of extract in one run within 2hr. On the basis of MS, IR, and 1H NMR analysis, the product of alkylamides was confirmed to be composed of hydroxy-α-sanshool, hydroxy-β-sanshool, and hydroxy-ε-sanshool. Furthermore, using the HSCCC product as external standard, alkylamide in the extract was determined to be in total of 21.41%, among which hydroxy-α-sanshool, hydroxy-β-sanshool, and hydroxy-ε-sanshool were in 15.69%, 4.76%, and 0.96%, respectively. © 2011 Copyright Taylor and Francis Group, LLC. 
alkylamide; HSCCC; hydroxy-alpha, beta and epsilon-sanshools; separation; tingling-taste; zanthoxylum 
IRIS
• Methanol (Non-Cancer)
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