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1133120 
Journal Article 
Stability-indicating HPLC method for determination of vitamins B-1, B-2, B-3 and B-6 in pharmaceutical liquid dosage form 
Thomas, S; Kumar, R; Sharma, A; Issarani, R; Nagori, BP 
2008 
Yes 
Indian Journal of Chemical Technology
ISSN: 0971-457X 
15 
598-603 
English 
A simple, selective, precise and stability-indicating high-performance liquid chromatographic (HPLC) method for determination of vitamins B 1, B2, B3 and B6 in pharmaceutical liquid dosage form was developed and validated. The chromatographic conditions comprised a reversed-phase C18 column (250 × 4.6 mm), 5 μ with a mobile phase consisting of a mixture of solution (0.015 M l-hexane sulphonic acid sodium salt, pH 3.0 ± 0.05) and methanol in gradient elution. The flow rate was kept at 1.5 mL/min. and the detection was carried out at 280 nm. The retention times of vitamins B3, B2, B 6 and B1 were 6.3, 15.1, 19.9 and 42.7 min. respectively. The linear regression analysis data for the calibration plots showed good linear relationship with coefficient of correlation values, r = 0.999 for vitamins B1, B2, B3 and B6 in the concentration ranges of 23.08-42.85, 9.61-17.84, 116.36-216.09 and 7.01-13.02 μg/mL respectively. The method was validated for precision, recovery and robustness. The vitamins undergo degradation under acidic, basic, peroxide, photochemical and thermal conditions. Statistical analysis proves that the method is reproducible and selective for the estimation of vitamins under study. As the method could effectively estimate the vitamins in presence of their degradation products, it can be employed as a stability-indicating method. 
Vitamin B-1,B-2, B-3 and B-6; HPLC; Stability indicating; Validation 
IRIS
• Methanol (Non-Cancer)
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